The prototyping model is a software development process that begins with requirements collection, followed by prototyping and user evaluation. Often the end users may not be able to provide a complete set of application objectives, detailed input, processing, or output requirements in the initial stage. After the user evaluation, another prototype will be built based on feedback from users, and again the cycle returns to customer evaluation. The cycle starts by listening to the user, followed by building or revising a mock-up, and letting the user test the mock-up, then back.
In the mid-1980s, prototyping was seen as the solution to the problem of requirements analysis within software engineering. Prototypes are any form of deliverables that model an application allowing users to visualize the application that is not yet constructed. Prototypes help users get an idea of what the system will look like, and make it easier for users to make design decisions without waiting for the system to be built.
Thursday, December 27, 2007
Tuesday, December 18, 2007
PCR optimization
PCR(polymerase chain reaction) can fail for various reasons, in part due to its sensitivity to contamination causing amplification of spurious DNA products. Because of this, a number of techniques and procedures have been developed for optimizing PCR conditions. Contamination with extraneous DNA is addressed with lab protocols and procedures that separate pre-PCR reactions from potential DNA contaminants. This usually involves spatial separation of PCR-setup areas from areas for analysis or purification of PCR products, and thoroughly cleaning the work surface between reaction setups. Primer-design techniques are important in improving PCR product yield and in avoiding the formation of spurious products, and the usage of alternate buffer components or polymerase enzymes can help with amplification of long or otherwise problematic regions of DNA.
PCR optimization
PCR(polymerase chain reaction) can fail for various reasons, in part due to its sensitivity to contamination causing amplification of spurious DNA products. Because of this, a number of techniques and procedures have been developed for optimizing PCR conditions.[10][11] Contamination with extraneous DNA is addressed with lab protocols and procedures that separate pre-PCR reactions from potential DNA contaminants.[4] This usually involves spatial separation of PCR-setup areas from areas for analysis or purification of PCR products, and thoroughly cleaning the work surface between reaction setups. Primer-design techniques are important in improving PCR product yield and in avoiding the formation of spurious products, and the usage of alternate buffer components or polymerase enzymes can help with amplification of long or otherwise problematic regions of DNA.
Tuesday, December 11, 2007
Television
Television (often abbreviated to TV, T.V., or more recently, tv) is a widely used telecommunication system for broadcasting and receiving moving pictures and sound over a distance from all around the world. The term may also be used to refer specifically to a television set, programming or television transmission. The word is derived from mixed Latin and Greek roots, meaning "far sight": far, and Latin vision, sight.
Since it first became commercially available from the late 1930s, the television set has become a common household communications device in homes and institutions, particularly in the First World, as a source of entertainment and news. Since the 1970s, video recordings on VCR tapes and later, digital playback systems such as DVDs, have enabled the television to be used to view recorded movies and other programs.
Since it first became commercially available from the late 1930s, the television set has become a common household communications device in homes and institutions, particularly in the First World, as a source of entertainment and news. Since the 1970s, video recordings on VCR tapes and later, digital playback systems such as DVDs, have enabled the television to be used to view recorded movies and other programs.
Thursday, December 06, 2007
Natural nuclear reactors
The natural nuclear reactors formed when a uranium-rich mineral deposit became inundated with groundwater that acted as a neutron moderator, and a strong chain reaction took place. The water moderator would boil away as the reaction increased, slowing it back down again and preventing a meltdown. The fission reaction was sustained for hundreds of thousands of years.
These natural reactors are extensively studied by scientists interested in geologic radioactive waste disposal. They offer a case study of how radioactive isotopes migrate through the earth's crust. This is a significant area of controversy as opponents of geologic waste disposal fear that isotopes from stored waste could end up in water supplies or be carried into the environment.
These natural reactors are extensively studied by scientists interested in geologic radioactive waste disposal. They offer a case study of how radioactive isotopes migrate through the earth's crust. This is a significant area of controversy as opponents of geologic waste disposal fear that isotopes from stored waste could end up in water supplies or be carried into the environment.
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